Mushroom Growing Substrate: Complete Guide (2024)

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Growing mushrooms starts with selecting the right mushroom growing substrate for your grow. The mushroom growing substrate is another word for the mushroom growing medium. This is what the mushroom mycelium grows through and it provides nutrition to the mushroom mycelium and subsequent fruiting mushrooms.

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You can think of a mushroom growing substrate just like soil for plants – some plants require special soil or enriched soil, while others don’t. In this guide, we will break down the different substrates that common growing mushrooms prefer.

Choosing a Mushroom Growing Substrate

The key part to understand is that every mushroom tends to grow best in certain types of substrates. For example, a King Oyster mushroom may grow best in hardwood sawdust, while a White Cap mushroom may prefer Manure.

As stated above, the nutrition of each substrate is what the mushrooms depend on to grow. This can be protein content, sugar content, potassium, fats, and nitrogen.

Another key component of the mushroom growing substrate is the water content. Many substrates require water as an added ingredient, but the amount of water is important. This can sometimes be 1/4 water as the volume of the ingredients, but it highly depends on the mushroom chosen.

You may have learned from other sources that sterilization of the substrate is important, and this cannot be understated. Using a sterilized growing medium is extremely important, otherwise you risk mold overtaking your substrate and ruining the mycelium. Always use safe and effective methods to sterilize your mushroom growing substrate, such as a pressure cooker.

Learn how to sterilize substrates in our guide here.

Mushroom Growing Substrate Examples

King Oyster Mushroom Substrate

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King Oyster mushrooms grow best on cellulose-rich mediums. This is usually going to be sawdust, straw, or a mix of the 2. Our best recommendation for king oyster substrate is the following:

  • Blend of hardwood sawdust (Amazon)
  • Supplement of 5-10% of wheat bran (Amazon)
  • 60-70% moisture content
  • a ph level of 6.5 after sterilization.
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These conditions and ingredients will allow the king oyster mushrooms to grow best. Other mediums can prove successful, but this blend is known to be highly effective.
Always take into consideration the other aspects of a well-controlled grow such as temperature of the grow room and lighting. While we can’t cover all of that detail in this guide, we have plenty of guides on that information on our site here.

Shiitake Mushroom Substrate

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Shiitake mushrooms grow best on wood-based mediums. Much like the king oyster mushroom, they prefer hardwood sawdust. Other mediums will work, but Shiitake mushrooms are more picky than Oyster mushrooms and it’s best to choose sawdust or other forms of ground wood. Here are the ingredients for Shiitake substrates:

  • Blend of hardwood sawdust (Amazon)
  • Supplement of 0-5% of wheat bran (Amazon)
  • 55% moisture content
  • a ph level of 6.5 after sterilization.

Portabello Mushroom Substrate

Portabello mushrooms prefer an alternative substrate of manure-based compost (Amazon). This will usually be a compost that is blended with manure. This should be easy to find, as many types of compost already come mixed with manure.

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You will still perform the normal steps of sterilization, and layer your manure-based compost into a wide cubic bin. Then, you’ll pour in some of your portabello spores and then leave the substrate to grow. This can be done indoors or outdoors, but given that compost/manure can be a bit messier than other substrates, outside is best.

White Button Mushroom Substrate

White Button mushrooms also prefer manure-based compost (Amazon) as their preferred growing medium. This will be a similar process to the above portabello guide.

An easy trick for growing white button mushrooms is to combine a 50/50 blend of manure and compost and blend it up. Now, with this substrate prepared, you can be assured you’re using the best substrate for white button mushrooms.

Conclusion

We listed a few of the most popular mushroom’s favorite growing mediums. These are some of the mushrooms that are easiest to grow at home. In the entire kingdom of mushrooms, there are so many mushrooms that require such special growing circ*mstances that you simply can’t grow them at home. This includes mushrooms that require a fresh forest burn to grow, or mushrooms that grow on dying trees.

Because of this, choosing easy-to-grow mushrooms is important, especially as a beginning grower. Choose a simple mushroom with an simple substrate and get started.

To really drive the point home, we highly recommend you take the sterilization of your growing substrate very seriously. Much of your hard work can go to waste if you don’t properly sterilize your mushroom growing medium with a pressure sterilization method.

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Mushroom Growing Substrate: Complete Guide (2024)

FAQs

What are the steps in mushroom substrate? ›

Contents
  1. STEP 1: Growing out a chosen mushroom culture on an agar filled petri dish.
  2. STEP 2: Transferring the mushroom culture (known as mycelium) onto sterilized grain.
  3. STEP 3: Expanding the grain jar exponentially to create grain spawn.
  4. STEP 4: Spawning the grain into a suitable substrate and fruiting container.

How much mycelium is enough? ›

The common standard of spawning is 8-10 liters, or 5-6 kilograms of mycelium per ton of phase 2 compost.

What is the best substrate for mushroom spawn? ›

If you have access to Rye, it will be your best choice most of the time. It's excellent at holding water (can hold more moisture than any other grain), and doesn't often burst or get mushy when processed.

What is the Uncle Ben method? ›

"Uncle Bens Tek" Grow Method

Uncle Ben's Tek, or Spiderman Tek is a user-friendly method for rapidly growing mushrooms. It utilizes pre-sterilized rice bags, eliminating the requirement for a costly pressure cooker. Wipe down all surfaces and tools with alcohol wipes or rubbing alcohol.

What is the easiest mushroom substrate? ›

Pretty much every mushroom growing resource I could find says that oyster mushrooms are the easiest variety for first time-growers, as they grow fast and can easily thrive in substrates made of things like coffee grounds and straw, making them relatively low maintenance.

How wet should mushroom substrate be? ›

Your substrate should be slightly acidic, with a PH between 5 and 6.5. (Some mushrooms, such as oyster mushrooms, can withstand a PH of up to 8.) A minimum moisture content of 50-70% is essential for your substrate.

What does healthy mycelium look like? ›

Spend time getting to know what your mycelium is supposed to look like so you can better identify unhealthy or contaminated patches. While mycelium is typically white and filamentous, contamination generally takes the form of green, blue, gray, or black patches or discolorations in your substrate.

What makes mycelium grow faster? ›

Humidity: High humidity levels (around 95-100%) are ideal for mycelium growth. 3. Air Exchange: Regular air exchange is necessary to provide fresh oxygen and remove carbon dioxide.

How often should you mist mycelium? ›

How often do I water my mushrooms? Using a spray bottle of clean tap-water, mist the exposed mycelium from your cut twice each day. Continue this process daily as you see your baby mushrooms begin to grow. Mushrooms like wet conditions, if you are running your AC or things look dry don't be afraid to add more water!

Why is Uncle Ben gone? ›

It comes after frequent accusations of racism against Uncle Ben's logo – an illustration of a black rice farmer – and name. In plantation-era US, white people in the southern states would often refer to a black man as 'uncle' to avoid using the more respectful 'mister'.

Is Uncle Ben healthy or not? ›

Uncle Ben's instant rice is a low-fat food that contains no cholesterol or saturated fat per serving, which makes it suitable for people with heart disease or diabetes.

What is the new name for Uncle Ben's? ›

As advertised on September 23, 2020, Mars Inc. replaced both the name "Uncle Ben's" along with the brand's historic logo depicting a well-dressed and bald black man in a bow tie; rebranding itself as simply "Ben's Original", with the new packaging becoming widely available in the United States from about June 2021.

What are the stages of mycelium? ›

The mycelium (the 'body' or 'roots' of the mushroom) grows through this substrate and excretes enzymes to convert it into an absorbable form. There are four basic stages to the life cycle of a mushroom: Spore germination, colonisation, fruiting, and sporulation.

What are the steps in fungiculture? ›

The six steps are phase I composting, phase II composting, spawning, casing, pinning, and cropping."

What are steps involved in the preparation of mushroom fungal culture? ›

The workflow summarizing the step-by-step protocol: (A) mother culture growing with no contamination on agar medium; (B) spawn preparation, inoculation of the spawn substrate (sterilized plant seeds), and incubation; (C) lignocellulose substrate preparation, sterilization, and inoculation with the spawn and incubation; ...

How do you prepare a substrate? ›

The substrates must be clean, crack-free, sound, dry, free of substances that may impair adhesion (such as grease, bitumen, dust, paint or adhesive residues, etc.) and must comply with the relevant local standards. The existing dirt, weathered layers and paint coatings of low strength should be mechanically removed.

References

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